AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: JUDITH-HAJNAL BARTHA-VARI, RENÁTA ELEKES-DARABONT, LAURA-EDIT BARABÁS, RÉKA BARABÁS.
DOI: 10.24193/subbchem.2021.1.13
Published Online: 2021-03-20
Published Print: 2021-03-30
pp. 165-178
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ABSTRACT: A new and efficient immobilization method of phenylalanine ammonia-lyase was obtained using hydroxyapatite (HAP) and hydroxyapatite - carbon nanotube, gelatin and chitosan - composites (HAP-CNT, HAP-GEL and HAP-CS) as support material. HAP and HAP composites were characterized by transmission electron microscopy (TEM), laser diffraction particle size analyzer and XRD measurements. The obtained nanobioconjugates were tested in the ammonia elimination reactions from L-phenylalanine (L-1). The reusability and the time-course profile of the immobilized enzyme preparation was also tested. While the biocatalyst obtained by using HAP-CS-15 as support material provided the highest conversion rate in the ammonia elimination reaction from L-1 (98,1% compared to 98,5% obtained using the non-immobilized enzyme), the most stable biocatalyst proved to be HAP-PAL, which maintained more than 80% of its initial activity even after 5 cycles of reuse.
Key words: hydroxyapatite, composites, carbon nanotubes, gelatin, chitosan, phenylalanine ammonia-lyase, immobilization, ammonia elimination.
