AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: MATEI-ȘTEFAN DOBRESCU, DUMITRANA IORDACHE, ANCA BUTIUC-KEUL.
DOI: 10.24193/subbbiol.2022.1.07
Article history: Received: 31 March 2022; Revised: 27 April 2022;
Accepted: 9 June 2022; Available online: 30 June 2022.
©2022 Studia UBB Biologia. Published by Babeş-Bolyai University.
pp. 131-142
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CRISPR (clustered regularly interspaced short palindromic repeats) is an immune system used by bacteria to defend themselves from different types of pathogens. It was discovered that this immune system can modify itself in specific regions called spacers due to previous interaction with foreign genetic material from phages and plasmids. Through our research, we have identified in different bacterial isolates CRISPR arrays belonging to the subtypes I-E (present in 42 samples) and I-F (present in 9 samples). The number of spacers in CRISPR arrays was also estimated based on the array length as a possible connection with the systems activity. Our results yielded arrays as small as 200 bp and as large as 1400 bp.
Keywords: CRISPR array; clinical isolates; pathogens; bacteria
