AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: VALENTIN ADRIAN KISS, LILIANA JARDA.
Published Online: 2021-06-30
Published Print: 2021-06-30
pp. 26-27
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ABSTRACT: Convolvulus persicus is a critically endangered species, endemic to the embryonic shifting dunes of the Caspian Sea and the Black Sea. The risk factors for the populations of C. persicus in Romania have an anthropic origin (Kiss and Szatmari, 2020). At the same time, natural risks are also present, such as the solidification of the sands. The efficient approach of plant conservation involves the combination of ex-situ and in-situ strategies, having as the main objective the maintenance of genetic diversity. In our case, ex-situ conservation presents a viable and even indicated alternative. In-vitro conservation has been conducted before by Holobiuc on this species using fragments of the stem or root (Holobiuc et al., 2015) but not seeds. The study aims to obtain an optimal protocol for ex-situ conservation using seeds, as well as the multiplication of plant in-vitro culture. Seeds from 20 individuals were used and kept over the winter at 18-20 °C, as well as a month at 4 °C. To initiate the in-vitro culture, the seeds were disinfected and inoculated into culture vessels containing 50 ml of MS solid medium supplemented with 20% sucrose. The infection rate after 30 days from inoculation is 8%. The germination rate is 1%. Only one of the seeds germinated, and after 20 days from germination, it was passed on a medium supplemented with phytohormones. In conclusion, we can say that germination of C. persicus is quite difficult and it is easier to induce in-vitro cultures from fragments of the stem or root as was done in Holobiuc’s study.
Key words: critically endangered, in-vitro, seeds, endemic, ex-situ conservation.
