AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI În partea de jos este prezentat rezumatul articolului selectat. Pentru revenire la cuprinsul ediţiei din care face parte acest articol, se accesează linkul din titlu. Pentru vizualizarea tuturor articolelor din arhivă la care este autor/coautor unul din autorii de mai jos, se accesează linkul din numele autorului. |
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STUDIA BIOLOGIA - Ediţia nr.1 din 2021 | |||||||
Articol: |
EVALUATION OF SELENIUM CONTENT IN BIOFORTIFIED ALLIUM SPECIES BY CHROMATOGRAPHIC MEANS. Autori: BOGDAN M. BOȘCA, AUGUSTIN C. MOȚ. |
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Rezumat: Published Online: 2021-06-30 Published Print: 2021-06-30 pp. 20 VIEW PDF FULL PDF ABSTRACT: Selenium is an essential trace element in animals and humans. It has chemical properties similar to sulfur. In plants, selenium is involved in several important biochemical routes, including antioxidative processes (Germ et al., 2007). In animals and humans, compared to other micronutrients, there is a much thinner line between the normal concentration and the toxic amount of selenium. The inorganic forms of selenium (selenate, selenite) present a much higher toxicity than its organic combinations (e. g. selenocysteine, selenomethionine) (Herrero Latorre et al., 2013). Thus, a sensitive method is required for detection of selenium species in biological samples. A distinct method is represented by the formation of piazselenol. This kind of complexes represent the product of reaction between an aromatic o-diamine and Se(IV) species, in acidic conditions. This study describes an HPLC-based optimized, sensitive and selective method for determination of selenium in biological samples. The analyzed Allium species were grown in our laboratory in a phytochamber. Before the analysis procedures, the biofortified Allium samples have been mineralized and pre-concentrated using solid-phase microextraction technique. This analytical step affords an excellent precision and accuracy as it removes the interferences form a typical biological sample, thus allowing a limit of detection as low as 0.5 ng/mL Se. Key words: Allium, biofortification, piazselenol, selenium determination |
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