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Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI
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Rezumat: In a drug discovery process from halophiles, Salinivenus iranica crude metabolites showed a great cytotoxic activity against breast cancer cell lines including MCF-7, MDA-MB-468, and MDA-MB-231. HPLC analysis and downstream MTT assays were done to purify the active component of the S. iranica metabolites. Moreover, FT-IR and LC-MS analysis were done to structural detection of the effector molecule of the selected metabolite. To find the effective molecule in S. iranica supernatant metabolite, the metabolite was fractionated into 31 fractions by four solvents: hexane, ethyl acetate, water, and butanol. The viability of human breast cancer cells was assayed in the presence of each fraction with the concentration of 100 µg/mL. The results showed that fraction number 6 of hexane had reduced the viability of all cell lines to 1% while the other 30 fractions had no significant effect on cell lines viability. The mass spectrophotometry determined that molecular weight (MW) of this fraction was about 1961.78 Da. In FT-IR analysis, three types of functional molecules were found in hexane fraction number 6. In 2950 – 2850 cm-1, Alkyl C-H Stretch was detected which are usually less useful in determining structure because of fairly ubiquitination. In 3500 - 3300 cm-1, Amine N-H Stretch was detected which is a primary amine, as primary amines produce two N-H stretch absorptions, secondary amides only one, and tertiary none. In 3550 – 3200 cm-1, Alcohol/Phenol O-H Stretch was detected. In conclusion, based on its unique features, the active competent of S. iranica metabolite could be a novel component against breast cancer and additional studies and full characterization of this effector molecule could result in a new drug with strong anti-cancer stem cell effects on breast cancer.
Keywords: breast cancer, drug discovery, LC-MS, Salinivenus iranica. |
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