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Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI
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Rezumat: Their ability to live in salt concentrations makes halophiles a good choice for a search for novel bioactive natural components with different medicinal applications. The role of metabolites from haloarchaeal strains belonging to different groups for application in cancer treatment is not well studied (Hou & Cui, 2018). A total of eight haloarchaeal metabolites have been evaluated for their anticancer potential against five human cancer cell lines of breast, lung and prostate cancers, while using a fibroblast cell line as a control. Downstream analysis including sphere and colony formation assay, invasion and migration assays, real-time PCR analysis, cell cycle, apoptosis assays and in vivo experiments were done to find the most potent metabolite with efficient anti-cancer activity. All archaeal metabolites investigated were found to be cytotoxic against one or more cancer cell lines and/or normal cells after 48 h with cell line specific activity. A metabolite from one strain has a potent cytotoxic effect on Du-145 and PC3 cell lines with an IC50 of 500 µg/ml without any effects on normal cells, and reduced sphere and colony formation ability of DU145 and PC3 cells with down-regulation of SOX2 gene expression. This metabolite affected the invasion and migration ability of these cell lines. It significantly increased both early and late apoptosis in the androgen-dependent PC3 cell line and caused tumor shrinkage in human prostate tumors in nude mice. The results of the present study indicated a major anti-cancer effect of metabolites derived from haloarchaeal strains.
Keywords: cancer, haloarchaea, sphere formation. |
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