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Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI
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Rezumat: The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources under aerobic conditions. In order to identify genes with potential importance in nitrogen assimilation and its regulation mechanisms, the global gene expression was analysed in different nitrogen sources, such as ammonium, nitrate and nitrogen starvation, by microarray technology. The results indicate that the absence of ammonium is the responsible factor for the expression of genes involved in nitrate assimilation pathway. Moreover, seven transcriptional regulators with significant differential expression have been identified: transcriptional regulator of ArsR family (arsR1, HFX_RS03970 and arsR2, HFX_RS03540), PadR family (padR, HFX_RS03340), and AsnC family (lrp, HFX_RS12185); GvpE transcriptional regulator (gvpE, HFX_RS08310); wecE pleiotropic regulatory protein (wecE, HFX_RS17500) and hypothetical regulator (HFX_RS17105). The gvpE gene and hypothetical regulator are up-expressed in nitrogen starvation and nitrate respect to ammonium, while arsR1 is down-expressed in the same conditions. The padR gene is down-expressed and the lrp gene is up-expressed in nitrogen starvation respect to ammonium. On the other hand, the arsR2 gene is up-expressed and the wecE is down-expressed in nitrate respect to ammonium. Microarray data were validated by qRT-PCR. To clarify the function of these seven regulators in the nitrogen and amino acids metabolisms, a meticulous bioinformatic analysis was carried out. This work represents a study of transcriptional profiles related to nitrogen assimilation and amino acids metabolism in extreme halophilic microorganisms, which allows the identification of transcriptional regulators.
Keywords: haloarchaea, microarray, qRT-PCR, transcriptional regulator.
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