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    STUDIA BIOLOGIA - Ediţia nr.1 din 2019  
         
  Articol:   TAT-FILAMENTS – NOVEL TYPE OF ARCHAEAL SURFACE STRUCTURES.

Autori:  ANNA GALEVA, ALEXEY SYUTKIN, MIKHAIL PYATIBRATOV, IGOR KIREEV, OLEG FEDOROV.
 
       
         
  Rezumat:  Here we describe Tat-filaments – a completely new kind of archaeal surface appendages isolated from haloarchaeon Haloarcula hispanica. The uniqueness of these structures is that they are formed of a protein subunit secreted using the Tat-pathway in contrast to all known archaeal surface structures secreted by the Sec-pathway. We isolated surface structures from H. hispanica (by PEG- mediated precipitation) and using electron microscopy we found along with archaella (10-11 nm in diameter) thinner filaments with a diameter of ~ 3 nm. On the SDS-electropherogram we observed an additional major protein band along with the archaellins. An increase in salinity of the growth medium results in an increase of the relative content of this protein and it correlates with an expansion in the number of thin filaments relative to archaella in the preparations. Mass spectrometry revealed that this extra protein band corresponds to the product of the hah_0240 gene, which encodes, according to the annotation, a hypothetical protein. Bioinformatic analysis showed that the HAH_0240 protein contains a twin-arginine motif used in the so-called twin-arginine translocation (Tat) pathway, a special secretion way for proteins in plants, Bacteria and Archaea. However, no evidence of the use of the Tat-pathway in the assembly of filamentary structures in Archaea has been described to this moment. The found filaments (Tat-filaments) are the first type of such structures. The role of the Tat-filaments still remains elusive, but our preliminary data suggest that these structures may be involved in biofilm formation and in cell adhesion to surfaces.

Keywords: archaeal surface structures, Haloarcula hispanica, Tat-filaments, Tat-pathway
 
         
     
         
         
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