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    STUDIA BIOLOGIA - Ediţia nr.1 din 2019  
         
  Articol:   CULTURABLE PROKARYOTIC DIVERSITY FROM SAMBHAR HYPERSALINE LAKE.

Autori:  SWAPNIL KAJALE, YOGESH SHOUCHE, NEELIMA DESHPANDE, AVINASH SHARMA. 		 
       
         
  Rezumat:  Sambhar Salt Lake is a large inland hypersaline lake in India situated at the eastern fringe of Thar desert, Rajasthan. This wetland is designated as Ramsar site due to its biotic importance and also known as the major salt producing site in India. High salinity, alkalinity and UV radiation makes this athalassohaline lake an unique natural ecosystem to explore the extremophilic microbial diversity. The present work investigated the culturable fraction of haloalkaliphiles from this less explored habitat using multiple methods. Sediment samples were collected from five different sites based on their locations and ionic strengths. Prokaryotic diversity was studied using various growth media and later identified using 16S rRNA gene sequencing. Whole genome squencing of potential isolates was carried out. A total of 447 isolates were identified belonging to 3 archaeal and 5 bacterial families. We also identified isolates belonging to 8 archaeal and 3 bacterial genera as potential novel taxa. Altogether the cultivation strategies used in this study contributed in capturing major haloarchaeal and halobacterial diversity with potential novel taxa which will enrich the microbial resource centres.

Keywords: athalassohaline, haloalkaliphile, Sambhar salt lake, 16S rRNA gene. 		 
         
     
         
         
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