Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI

În partea de jos este prezentat rezumatul articolului selectat. Pentru revenire la cuprinsul ediţiei din care face parte acest articol, se accesează linkul din titlu. Pentru vizualizarea tuturor articolelor din arhivă la care este autor/coautor unul din autorii de mai jos, se accesează linkul din numele autorului.

 
       
         
    STUDIA BIOLOGIA - Ediţia nr.1 din 2019  
         
  Articol:   “METAVIROMICS ASKS, METAVIROPROTEOMICS ANSWERS”.

Autori:  MARÍA DOLORES RAMOS-BARBERO, JOSEPH CHRISTIE-OLEZA, JUAN RAMÓN HERNÁNDEZ-FERNAUD, MANUEL MARTÍNEZ-GARCÍA, FERNANDO SANTOS, JOSEFA ANTÓN. 		 
       
         
  Rezumat:  Viruses in hypersaline environments can reach up to 1010 particles per ml (DiMeglio et al., 2016). Therefore, these systems constitute a perfect scenario for virus-host interactions studies. Despite their simplicity, viral genomes can contain a high proportion of genes with unknown functions (coding for hypothetical proteins, HPs). Based on the fact that proteins in virions are mainly structural, we have applied a protocol for the study of viral proteins directly from the environment: the metaviroproteomes. We analyzed two metaviroproteomes from two ponds of Bras del Port salterns (Spain) and developed a new pipeline for the search of viral peptides against the database of proteins (DB) constructed from their corresponding metaviromes. This procedure has allowed us to increase the percentage of peptides identification respecting to the previously reported protocol (Brum et al., 2016), and to associate many peptides to HPs-coding ORFs, now re-annotated as structural genes. Also, a fraction of peptides did not match against the DB as a consequence of the great metagenomics anomaly (Ramos-Barbero et al.., 2019), but they did match against non-assembled reads. Finally, the microdiversity contained within the structural peptides was analyzed throughout a large set of halometaviromes as a way to explore if it can be on the basis of expanding (or maintaining) the halovirus-host ranges.

Keywords: hypothetical protein, metavirome, metaviroproteome, virus 		 
         
     
         
         
      Revenire la pagina precedentă