AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: VICTORIA CRISTEA, MAGDALENA PALADA, LILIANA JARDA, ANCA BUTIUC-KEUL.
The preservation of endangered and/or threatened plant species by biotechnological methods as in vitro culture is a complementary alternative of in situ conservation. Several individuals of Dianthus spiculifolius Schur from two distinct and spatially separated populations, from Hăşmaş and Vlădeasa Mountains (Romania) were preserved by in vitro culture. Following several stages of in vitro culture as culture establishment, stabilization, stable culture multiplication, in vitro rhizogenesis and outdoor acclimatization, an optimal protocol for conservation and acclimatization was achieved. Four culture media variants were used for different culture stages. During the stable culture multiplication stage, after 60 days of culture, a maximum of 104.8 new shoots/uninodal explant for the Vlădeasa Mt. population and 24.9 new shoots/explant for the Hăşmaş Mt. population were obtained. Nodes explants, horiozontally inoculated generated a very high number of shoots (maximum 345.8 shoots). The rhizogenesis ratio was 10 roots/explant in population from Hăşmaş Mt. and 5.3 in population from Vlădeasa Mt. The vitroplants were successfully acclimatized and they were cultivated in a special outdoor rocky area. Keywords: acclimatization, endangered, endemic plant, explant type, micropropagation.
