AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: TÜNDE-ÉVA DÉNES, IMOLA MOLNÁR, ELENA RAKOSY-TICAN.
Potato late blight is the most devastating disease of potato caused by the oomycete pathogen, Phytophthora infestans a very virulent and adaptable pathogen. The wild potato species, Solanum bulbocastanum (2n, 2x24), is highly resistant to all known races of Phytophthora infestans, even under intense disease pressure. Oomycete infects foliage and tubers alike, resulting in 16% annual yield loss. Because potato varieties and S. bulbocastanum are sexually incompatible somatic hybrids were produced by protoplast electrofusion. Putative somatic hybrid shoots were selected through flow cytometry, only the hexaploid plants being regenerated. The BC1 and BC2 progenies were obtained by crossing hybrids with different potato cultivars. The ploidy level of somatic hybrids and their derived BC progenies maintained in vitro is presented here after chromosome counts and analysis. The chromosomes were stained with 4’6 diamidino-2-phenilyndole (DAPI) and numbered under epifluorescent microscopy (Olympus BX-60). Micromeasure 3.3 software was used to measure chromosomal parameters from electronically captured images and assemble ideograms of parents, somatic hybrid clones and BC progenies. Acknowledgements. We express our gratitude for funding to the project CNCS PNII-ID-PCE-2011-3-0586.
