AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI
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Autori: SÁRA PÓLYA, EDINA POCZKODI, ANNA POZSONYI, GÁBOR TÓTH, LÁSZLÓ TAMÁS.
The main aim of the project we have been working on is to produce edible vaccines in selection marker gene free transgenic barley endosperm. To reach these goals a system has to be established to generate proper transgenic barley lines. The most suitable epitopes of a particular antigen can be quickly selected through the expression of several molecules in plant tissue culture, followed by the appropriate functional studies. To build up the above mentioned system transformation cassettes have been assembled and tested. Results are presented here on the development of the barley heterologous protein expression system. Antigenic proteins which trigger an effective mucosal immune response can also be produced in transgenic plants. This plant derived edible vaccines are not only cheaper and safer than recently used subcutaneous vaccines but they are considered to be more effective. Edible vaccines are particularly effective against those pathogens which enter trough the mucosal membrane. The cholera toxin B subunit (CTB) as a strong mucosal adjuvant was used to elicit the mucosal immune response, because CTB has an effective adjuvant activity as a carrier protein for genetically fused unrelated proteins. Genes of potential immunogenic proteins or epitopes are able to fuse easily in this cloning system with the gene of the adjuvant protein. Our intention is to develop a high throughput transient expression system to test and investigate these potential antigenic proteins. Both Agrobacterium mediated and biolistic methods are able to use for barley cells transformation. To achieve high expression level in short time the maize ubiquitin promoter, which is a strong constitutive promoter in cereal was used to drive the protein expression in the barley tissue. The produced proteins can be used for small scale functional studies in immunology assays because they have desirable biochemical and immunological properties. For animal feeding experiments and biopharmaceutical industrial applications endosperm specific expression system has to be established. The aim is to create selection marker gene free transgenic barley plant. The vaccine genes are driven by a wheat HMW glutenin promoter, which is strictly endosperm specific. The genes were chosen for preliminary studies can be cloned into the stable transformation vector in one single step.
