AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: LÁSZLÓ TAMÁS, CSABA ÉVA, ESZTER KISS, FLÓRA TÉGLÁS, KLÁRA MÉSZÁROS, LÁSZLÓ LÁNG.
One of the main concerns regarding GM plants is the presence of the selection marker gene in their genome. Although several non-antibiotic and non-herbicide based selection systems exist, but they are the most frequently used, which raised the fear in the public. The biggest problems addressed by the green movements are the so called “escape of these selection marker genes into the environment causing negative effects on it. The other problem with the most widely used methods modifying the plant genome is the random insertion of the “foreign” gene into it and as a consequence it may generate unpredictable expression patterns. To reduce some of the concerns and make the gene technology approach acceptable by the society scientists have to address all these issues. Selection marker genes have to be chosen carefully or have to be removed when they are no longer necessary in the genome. The genes for modifying the physiological characteristics of the plant or the property of the products made of plant tissue have to be chosen prudentially introducing genes from the same species or from the same family. To avoid random insertion for example site-specific genome editing is powerful and highly desirable methodology. Both site-specific recombination and restriction enzyme mediated gene targeting are feasible approaches in genome modification of plant cells. A method based on the widely used Cre/lox recombination system has been developed in or laboratory to produce selection marker free transgenic barley plants. The inducible recombination system is under the control of a cold responsible promoter. It has been demonstrated that the system works properly cutting of the unnecessary DNA fragment from the barley genome. The remaining mutated loxP site provide an option for site specific insertion of any desirable DNS fragments to engineer specific properties of either the plants or plant based products.
