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    STUDIA BIOLOGIA - Ediţia nr.1 din 2007  
         
  Articol:   CHLORORESPIRATION STUDY ON ISOLATED CHLOROPLASTS FROM THE GREEN ALGA MOUGEOTIA SP., STRAIN AICB 560.

Autori:  VICTOR BERCEA, CĂTĂLINA VASILESCU, BOGDAN DRUGĂ, NICOLAE DRAGOŞ.
 
       
         
  Rezumat:  The kinetics of plastoquinone oxidation in darkness at isolated chloroplasts from Mougeotia in the presence of DCMU and of some mitochondrial respiratory inhibitors was studied. In the presence of the acceptor methyl viologen, DCMU conducted to the increase of the available plastoquinone pool and the fraction Q-A decreased. N-propyl gallate sustained the oxidation of plastoquinone in darkness, and Q-A increased at 64%. Salicylhydroxamic acid (SHAM) sustained the plastoquinone oxidation in darkness, and Q-A decreased. Rotenone inhibited the plastoquinone oxidation in darkness, and Q- decreased at 70%. In the presence of the acceptor ferricyanure, DCMU did not produce changes in plastoquinone pool. PG and SHAM did not change the plastoquinone redox state, rotenone sustained the plastoquinone oxidation in darkness, while Q-A decreased. It was observed that the partial reduction of plastoquinone pool was accompanied by changes in the initial fluorescence. The results showed the presence of respiratory type of electron transfer from NAD(P)H to oxygen in chloroplasts, mediated by plastoquinone pool involved in the photosynthetic electron transfer chain, process driven by chlororespiration.  
         
     
         
         
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