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	STUDIA BIOLOGIA - Ediţia nr.1 din 2007

	Articol:	THE KINETICS OF PLASTOQUINONE RE-OXIDATION IN DARKNESS, IN THE CHLOROPLASTS ISOLATED FROM THE GREEN ALGA MOUGEOTIA SP., STRAIN AICB 560. Autori: VICTOR BERCEA, BOGDAN DRUGĂ, CĂTĂLINA VASILESCU, NICOLAE DRAGOŞ.


	Rezumat: The plastoquinone re-oxidation in darkness in the presence of the methyl viologen and ferricyanure acceptors in the Mougeotia isolated chloroplasts was studied. In order to stop the mitochondrial respiration there were used certain inhibitors which act on the electrons transport in the same way like n-propyl gallate (PG), salicylhydroxamic acid (SHAM) and rotenone in the presence of diurone (DCMU) do. In the presence of the methyl viologen acceptor, DCMU and SHAM have enhanced the amount of re-oxidized plastoquinone, while the quantity of Q-A has decreased. Propyl gallate has generated the stimulation of the plastoquinone re-oxidation, but the amount of Q-A has been decreased. In the presence of ferricyanure, DCMU, propyl gallate, SHAM and rotenone have inhibited the plastoquinone re-oxidation in darkness, and the reduced acceptors quantity has been increased. The plastoquinol re-oxidation in darkness followed by the reduction by light has caused the initial fluorescence change. In the presence of DCMU and artificial electrons acceptors, the supply of quinone acceptors becomes re-oxidized in darkness because of the cyclic electrons chain around PS I where the chlororespiration and the Ndh complex are involved, this influencing directly the photosynthesis.




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