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    STUDIA BIOLOGIA - Ediţia nr.1 din 2005  
         
  Articol:   CHANGES IN CHLOROPHYLL FLUORESCENCE DURING THE GREENING OF ETIOLATED LEAVES.

Autori:  LASZLO FODORPATAKI, ISTVAN-ZOLTAN VASS. 		 
       
         
  Rezumat:  Etiolated leaves of barley and maize plantlets were exposed to two different photon flux densities (150 and 600 μM m-2s-1) and the parameters of the induced chlorophyll fluorescence were registered every 24 hours during the greening of leaves. Variations in the ground fluorescence, the maximal fluorescence, the variable fluorescence, the variable/maximal fluorescence ratio, as well as in the time needed to reach the maximal fluorescence were determined in order to get a better insight in the processes ralated to the gradual transformation of etioplasts into functional chloroplasts during the greening of dark-grown leaves. Deductions were made regarding the development of the light-harvesting pigment-protein complexes and their capacity to absorb light. Variations of the potential quantum yield efficiency of photosystem II were monitored during the greening process, and the electron transport capacity of the acceptor side of PS II was evaluated on the base of the reduction state of the primary quinone acceptor pool of the thylakoid membranes developed in the young chloroplasts. The results demonstrate the usefulness of chlorophyll fluorescence parameters in getting new insights into the development of the primary photosynthetic processes during the greening of etiolated leaves. The significance of the results was evaluated with the one-way ANOVA and with the Tukey test. The first day of exposure to light (disregarding the photon flux density) is crucial in the onset of processes involved in the light reactions of photosynthesis and in the increment of the potential quantum yield efficiency of photosystem II.  
         
     
         
         
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