Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI

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    STUDIA BIOLOGIA - Ediţia nr.1 din 2005  
         
  Articol:   COMPARISON OF THE EFFECT OF SUCCUSSED AND UNSUCCUSSED DILUTIONS OF SOME STIMULATING AGENTS IN THE RELEASE OF REACTIVE OXYGEN SPECIES BY THE HUMAN POLYMORPHONUCLEAR LEUKOCYTES.

Autori:  MARIANA STEJAR, CORNELIU TARBA.
 
       
         
  Rezumat:  Despite serious reservations that exist with regard to the efficacy of the homeopathic treatments, many clinical and laboratory physicians as well as biomedical investigators with a strong physico-chemical background continue to pay a special attention to this problem. Aware of the fact that the main obstacle in accepting the idea of homeopathic medicine resides in the difficulty of proposing a plausible explanation to the way in which a substance found in an infinitesimal concentration is capable of producing effects, extremely sophisticated hypotheses (from a physico-chemical point of view) have been lately elaborated, involving as a support for information storage and transmission the complex structure of water in conjunction with the specific methods (succussion/dynamization) by which homeopathic preparations are obtained. Since the essence of such hypotheses relies on the cooperative interactions existing among the water molecules, the homeopathic effects (so far documented mostly at the level of the human organism) should be present and measurable at different structural/functional levels by in vitro objective biophysical-biochemical assays, which exclude psychological effects. If the homeopathic phenomena can not be evidenced, e. g., at the molecular/cellular level, the above-mentioned hypotheses are not tenable, regardless of how well articulated they may appear. In order to test in vitro the homeopathic effect, we measured the concentration of the reactive oxygen species (ROS) liberated in the incubation medium during the confrontation of the polymorphonuclear (PMN) leukocytes with stimulating factors, such as concanavalin A (ConA) and phorbol myristate acetate (PMA). These stimulating agents were diluted either conventionally or using homeopathic procedures (succussion/dynamization) while the reduction of cytochrome c (cyt c), measured as the absorbance increase at 550 nm, was chosen as an assay for ROS presence. The incubation medium (1. 5 ml), basically made of Hanks buffer, usually contained 106 cells/ml, 1 mg/ml cyt c and different conventional or homeopathic dilutions of ConA or PMA. By defining a stimulation index as (A A0)/A0, where A is the absorbance at 550 nm in the stimulated sample and A0 is the absorbance in the control sample, we obtained a very useful index for our quantitative evaluations. A similar approach was used in comparing directly the effects of the two types of dilutions (homeopathic and conventional) by calculating the percent differences between the response of paired samples, to which the paired t test was applied for establishing the statistical significance. Although we made a very large number of measurements, the number of pairs for each dilution varying between 3 and 13, there were no statistically significant differences in the case of the high and ultrahigh dilutions tested (1041030).  
         
     
         
         
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